Showing posts with label Genetic Engineering. Show all posts
Showing posts with label Genetic Engineering. Show all posts

Sunday, 20 October 2013

Genetic Engineering

Genetic engineering as a field emerged with the dawn of molecular biology when James Watson and Francis Crick deciphered the three dimensional structure of DNA. Several decades have passed since then, and today whole genome sequences of several different organisms across kindgoms have been deciphered giving rise to a wealth of information that is only a computer terminal away.

The book, while having only five chapters, covers a wide range of topics in genetic engineering of microorganisms, plants and animals. Specifically, it covers both the natural and social sciences. In the natural sciences topics ranging from the genetic engineering of microorganisms to produce antibiotics, the gene targeting and transformation in plants, the generation of marker-free plants in response to biosafety concerns, as well as the generation of transgenic animals and those derived through cloning are covered. In the social sciences, the issue of ethics in biotechnology and the role of the media in reporting around the cloned sheep, Dolly are discussed. Interestingly the application of genetic engineering to plants and animals generates controversy compared to the same application in microorganisms. Perhaps some of that controversy is due to the actual use of the end product, in the case of plants, food, and in the case of animals, even more so, as the reality of cloning animals conjures up fears that humans are about to be cloned even though cloning is not a transgenic process. This then brings on the whole issue of ethics in science, given the technological leaps and bounds of genetic engineering within the last decade compared to many before that. The book also highlights the role of the media in perpetrating the fear associated with the unknown. Clearly the role of the media should be one of responsible reporting with the goal of educating the public and not that of engendering fear.



Friday, 12 April 2013

Principles of Gene Manipulation, 6th Edition

As in past editions, the basic philosophy of this extremely popular text remains to present the principles and techniques of gene manipulation in sufficient detail to enable the non-specialist reader to understand them. However, in the three years since the previous edition, molecular biology has seen a dramatic increase in available techniques and applications. Every chapter in this new edition has been extensively revised or rewritten to take these developments into account. 

Principles of Gene Manipulation provides an excellent introduction to the area of genetic engineering of plants, animals and microbes for advanced level undergraduates, with a basic understanding of genetics. This classic textbook has been substantially updated and revised to reflect the rapid advances that have been made in the core technologies in the seven years since the last edition. Furthermore, to put these technologies into context, the final chapter has been structured into six themes: 
  1. nucleic acids as diagnostic tools
  2. new drugs and new therapies for genetic diseases
  3. combating infectious disease
  4. protein engineering
  5. metabolic engineering
  6. modern plant breeding

Thursday, 11 April 2013

Gene Cloning and DNA analysis: An Introduction 6th Edition


During the four years since publication of the Fifth Edition of Gene Cloning and DNA Analysis: An Introduction there have been important advances in DNA sequencing technology, in particular the widespread adoption of high throughput approaches based on pyrosequencing. Inclusion of these new techniques in the Sixth Edition has prompted me to completely rewrite the material on DNA sequencing and to place all the relevant information—both on the methodology itself and its application to genome sequencing into a single chapter. This has enabled me to devote another entire chapter to the post-sequencing methods used to study genomes. The result is, I hope, a more balanced treatment of the various aspects of genomics and post-genomics than I had managed in previous editions. A second important development of the last few years has been the introduction of real-time PCR as a means of quantifying the amount of a particular DNA sequence present in a preparation. This technique is now described as part of Chapter 9. Elsewhere, various additions, such as inclusion of topoisomerase-based methods for blunt end ligation in Chapter 4, and generally tidied up parts of chapters that had become slightly unwieldy due to the cumulative effects of modifications made over the 25 years since the First Edition of this book. The Sixth Edition is almost twice as long as the First, but retains the philosophy of that original edition. It is still an introductory text that begins at the beginning and does not assume that the reader has any prior knowledge of the techniques used to study genes and genomes.