As proteomics
technologies are reaching a plateau in the number of proteins that can be
resolved and detected, pre-fractionation steps have become essential to
increase the depth of proteomic analysis. So far, many pre-fractionation steps
have been based on chromatography methods where the proteins are separated
according to their individual physicochemical properties. Subcellular
fractionation methods proved to be very potent protein pre-fractionation steps:
they allow the representation of low abundance proteins, and they can be
combined with chromatography steps. Moreover, as the isolated subcellular components
also represent functional units, subcellular fractionation allows the proteomic
analysis of protein subsets that are functionally related in a biologically relevant
manner. The first three sections of this volume deal with different levels of
subcellular organization that also correspond to specific methodological approaches.
0 comments:
Post a Comment