Because
of the huge potential of human embryonic stem (hES) cells, especially the newly
developed human induced pluripotent stem (hiPS) cells, in disease treatment and
life quality improvement, enormous efforts have been made to develop new
methodologies to translate lab discoveries in stem cell research into bed-side
clinical technologies. In Human
Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation
Protocols, experts in the field present a comprehensive collection of
protocols designed for labs around the world. The topics covered in this
detailed volume include techniques used for maintenance of hES and iPS cells in
either small or large scale, techniques for directing hES and iPS cell lineage
specification, techniques for enhancing the maturity of differentiated hES and
iPS cells within three-dimensional scaffolds, techniques for reprogramming
adult cells into iPS cells, techniques for generating patient-specific iPS
cells, and techniques for translating hES and iPS cell research into new
therapies. Chapters include lab ready protocols with tips on
troubleshooting and avoiding known pitfalls.
Wide-ranging and
authoritative, Human Embryonic and Induced
Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students
who wish to explore these areas and transform their discoveries into the next
generation of regenerative medicine and tissue engineering technologies.
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