Immunocytochemistry
of plant cells has come a long way from the first review on this subject by
Bruce Knox in the early 1980s. In that early review, our only tools were
fluorescein-labeled antibodies for light microscopy and ferritin-labeled
antibodies for electron microscopic observation. Frankly, in many of these
early localizations the resolution of the tissue or the specificity of the
labeling left much to be desired.
Immunocytochemistry
of Plant Cells is the first book exclusively dedicated to this topic. The first
and largest portion of the book is concerned with a group of proven protocols
and variations on these protocols that might prove useful, many developed or
modified in the author's laboratory. The second portion of the book covers the
studies that have been published previously on each of the plant organelles.
Numerous state of the art micrographs from researchers around the world are
included to demonstrate typical results.
This book is
organized essentially into two sections. The first chapter gives what we
consider general protocols that work well on a variety of tissues and
organelles, but also a number of variations that one might try in order to
obtain a successful localization. Most of these were developed when the more
standard protocols failed. The second portion of the book reviews by organelle
of those techniques that may work better with that particular organelle, what
unique immunocytochemical techniques can be used, and a review of some of the
more important studies on that organelle. Some of the chapters also address the
questions that are still outstanding and which could benefit from
immunocytochemical studies.
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