Methods in Molecular Biology : Lipoproteins
and Cardiovascular Disease: Methods and Protocols is a compendium of
advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and
vascular biology research. Lipoprotein,
atherosclerosis, and vascular biology studies present unique challenges to the molecular biologist. The
lipid-rich and otherwise challenging nature of many key tissues complicate the isolation of high-quality RNA for gene
expression analysis, for example, and
the unique nature of lipoproteins and their biological effects has engendered
unique methodologies. To date,
no volume has yet encompassed these lipoprotein-centered cutting edge methods in molecular biology.
This book brings
together in a single volume an updated set of protocols and strategies for
methods now driving advances in lipoprotein and atherosclerosis research, along
with classical methods that are still widely used. The chapters are written for
researchers at any level, from graduate students to established investigators
with no prior experience in the described techniques, and may be of interest to
molecular biologists outside the lipoprotein field using similar techniques. Of
particular interest to readers are methods chapters on quantitative real-time
PCR, microarrays, RT-PCR laser capture microdissection, and tissue-speci fi c
gene overexpression, knockout, and knockdown methodologies, including AAV as a
liver-directed gene delivery vehicle. Special topics include an overview of
next-generation and third-generation sequencing, antisense technology,
chromatin immunoprecipitation, streamlined LCAT activity assays, and native HDL
subpopulation analysis. Updated methods for 5 ′ and 3 ′ RACE cloning of
full-length cDNAs and Northern analysis have been added. Overviews, strategic
considerations, and background information are included for particularly novel
or complex methods.
This edition
complements its classic predecessor, “Lipoprotein Protocols,” edited by Jose
Ordovas, by incorporating cutting-edge methodological advances developed over
the past decade. The two volumes together provide a complete, up-to-date set of
methods for any researcher with an interest in lipoproteins and their
biological effects.
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