Despite being
known and studied for years, peptides have never before attracted enough attention
to necessitate the invention of the term “Peptidomics” in order to specify the study
of the complement of peptides from a cell, organelle, tissue or organism. This
volume presents a comprehensive range of analytical techniques for analysis of
the peptide contents of complex biological samples. The emphasis is often on
higher throughput techniques, suitable for the analysis of large numbers of
peptides typically present in the peptidomes or other complex biological
samples. A wide range of methods is presented, covering all stages of
peptidomic research including, where applicable, organism handling, tissue and
organ dissection, cellular and subcellular fractionation, peptide extraction,
fractionation and purification, structural characterisation, molecular cloning
and sequence analysis. In addition to this, a selection of methods suitable for
quantification, display, immunochemical and functional analysis of peptides and
proteins are presented. The methods and techniques covered in this volume
encompass a number of species ranging from bacteria to man and include model
organisms such as Caenorhabditis elegans, Drosophila melanogaster and
Mus musculus. Strong emphasis is placed on data analysis, including mass
spectra interpretation and in silico peptide prediction algorithms. Where relevant,
the peptidomic approaches are compared to the proteomic methods.
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