The
introduction of monoclonal antibodies revolutionized immunology. The
development of human monoclonal antibodies was inspired primarily by the
enormous clinical benefits promised by these reagents which can be used as
anti-inflammatory reagents, anti-tumor reagents and reagents for passive
immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and
Protocols presents
technical protocols of cellular and molecular methods for the production,
purification and application of human monoclonal antibodies, as well as review
articles on related topics of human monoclonal and polyclonal antibodies.
Written in the successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls.
Authoritative
and easily accessible, Human Monoclonal Antibodies: Methods and
Protocols seeks to serve
both professionals and novices with its well-honed methodologies which will
prove invaluable in a clinical setting.
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