With the advent
of proteomics came the development of technologies, primarily mass spectrometry,
which allowed high-throughput identification of proteins in complex mixtures.
While the mass spectrometer resides at the heart of proteomics, its ability to characterize
biological samples is only as good as the sample preparation and data analysis tools
used in any study. Not only has proteomics increased our capacity to identify
proteins, it has enabled other characteristics of proteomes to be measured. Of
utmost interest has been the development of techniques for measuring
posttranslational modifications such as phosphorylation and glycosylation.
Since the amount of any specific protein within a cell is important to its
function, methods to quantitate protein levels have also been developed. These
quantitative methods include both label-free approaches and those that utilize
stable isotopes incorporated both during cell growth or added via a chemical
reaction once the proteome is extracted from the cell.
The purpose of
this book is to provide the student and researcher in the fields of Biochemistry,
Biomedicine, Molecular and Cellular Biology, and Bioinformatics a detailed description
of many of the different sample preparation and data analysis tools used in proteomics
today. The editors are indebted to each of the authors for providing their time
and expertise in making this edition an invaluable resource to anyone involved
or interested in proteomics.
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